Cell-based glycan arrays
WebJul 25, 2024 · The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. ... WebJun 3, 2024 · Using this "cell-based glycan array", recombinant lectins can be rapidly screened to determine specific glycan structures required for binding in living human cells (Büll et al., 2024). One study ...
Cell-based glycan arrays
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WebAug 4, 2024 · Cell-based glycan arrays enable testing of glycan binding directly on the surface of CHO cells by flow cytometry. Using recombinant glycosyltransferases, sialic acid, and Fuc, the small number of ... WebApr 23, 2024 · Probing the binding specificities of human Siglecs by cell-based glycan arrays. Proceedings of the National Academy of Sciences , 2024; 118 (17): e2026102118 DOI: 10.1073/pnas.2026102118 Cite This ...
WebThe cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which … WebApr 23, 2024 · Printed arrays of synthetic glycans and cell-based arrays have revealed that the individual Siglec family members have unique and partially overlapping binding specificities for distinct 'healthy ...
WebAug 1, 2024 · The closely timed development of cell-based glycan arrays and glyco-genomic profiling methods has been another major theme in recent years. There are … WebJan 25, 2024 · Independent cell-based glycan array studies have similarly shown that Siglec-7 preferentially binds O-linked glycans . However, our results do not preclude the possibility that gangliosides may be significant ligands for Siglec-7 in other select cell types, in particular neuronal cells that express such antigens at an unusually high density ( 36 ).
WebApr 23, 2024 · The cell-based glycan array enables further dissection of underlying structural requirements for binding using our sublibrary engineering strategy to dissect …
WebJun 3, 2024 · This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of … sprache marathiWebApr 8, 2024 · A prime example is live cell proximity-based tagging, which offers an impressive route to elucidate the carbohydrate targets of glycan-binding proteins 27,28,29. Applying this approach to viral ... shenzhen a-faith technology co. ltdWebThe 100 synthetic glycans featured in the Glycan Array 100 are the most frequently identified structures showing important binding function in the literature. For example, … shenzhen afkmed co. ltdWebOct 24, 2024 · A lectin array-based method can be used for rapid analysis of glycosylation profiles of glycoproteins and mammalian cell surfaces . The method is based on binding an intact glycoprotein to the arrayed lectins, resulting in a characteristic fingerprint that is highly sensitive to changes in glycan composition. sprache manipulationWebJul 1, 2024 · We previously used the cell-based glycan array platform to dissect the binding specificities of two Siglec-like binding regions (BRs) of serine-rich repeat adhesins expressed by oral streptococci ... shenzhen aerospace dongfanghong satellite ltdWebMay 6, 2024 · The information provided by glycan arrays, a glycan-binding profile for a receptor, is a critical starting point for downstream fundamental applications such as … shenzhen afternoon teaWebFeb 28, 2024 · Design and validation of cell-based glycan array strategy. As proof-of-principle, we used the CHO glycosylation mutant Lec2 cells 12 to construct in-solution, cell-based glycan arrays displaying ... We would like to show you a description here but the site won’t allow us. shenzhen aehealth technology co. ltd